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. 2012 Apr 19;11(8):527–539. doi: 10.1074/mcp.M111.015230

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 3. — Effect of normalization and integration procedures on the quantitative results after SDS-PAGE fractionation. A, histograms of CVs for 1) nontransformed protein PAI values calculated on raw data, 2) normalized protein PAI values transformed with a global correction factor (ratio of summed PAI values for all proteins along each migration lane in replicate experiments), or 3) protein PAI values calculated from normalized XIC signals for each group of matching gel bands. B, histograms of CVs for protein PAI values calculated after normalization of XIC signals in matching gel bands, taking into account only the fraction with the best PAI for proteins identified in several gel bands, or after integration by summing PAI values on three consecutive gel bands near the best intensity fraction.