Fig. 4.

Experimental design and identification results of the large scale quantitative proteomic study of endothelial cells. Three independent biological experiments were performed by stimulating HUVECs with inflammatory cytokines in culture (either a combination of TNFα and IFNγ or IL1β). Total cell lysates from control and stimulated samples were loaded and fractionated on six parallel gel lanes and cut into 12 gel [GRAPHIC]bands. The table indicates the number of proteins identified for each gel lane, and the total number of proteins identified and quantified in each experiment, as well as the number of proteins detected as differentially expressed.