Table I. Statistics of peptide migration across the SDS-PAGE fractions.
Peptide apex count distribution indicates the number of peptide precursor ions that were detected at their maximal intensity in the same matching fractions across all three replicates (one fraction), in the same fraction in only two replicates (two fractions), or in different fractions in the three replicates (three fractions). Peptide apex distance distribution illustrates the maximal gap between apex fractions when peptides were detected in nonmatching fractions across the replicates.
| Peptide apex | No. of precursor ions |
|
|---|---|---|
| Three migration lanes | Three injections of one lane | |
| Count distribution | ||
| One fraction | 33,932 | 34,769 |
| Two fractions | 1,402 | 364 |
| Three fractions | 91 | 0 |
| Distance distribution | ||
| 0 | 33,932 | 34,769 |
| 1 | 1,269 | 312 |
| 2 | 53 | 22 |
| 3 | 21 | 10 |
| 4 | 12 | 4 |
| 5 | 11 | 1 |
| 6 | 10 | 3 |
| 7 | 9 | 6 |
| 8 | 54 | 1 |
| 9 | 38 | 4 |
| 10 | 15 | 1 |
| 11 | 1 | 0 |