Figure 2. Total and surface amine densities of nanoparticles aminosilanised via TPRE and in water.

(a); 5′-NH₂dC₆dT₂₅ oligonucleotide grafted and hybridized 5′FdA₂₅ complementary oligonucleotide (b); hybrid capture using bacterial genomic DNA. PCR amplification of 566 bp bacterial 16S rRNA gene fragment (c1) and 310 bp pfkB-thrS intergenic region from Salmonella enteric (c2). Abbreviations: M, 100 bp DNA molecular weight ladder (New England Biolabs Inc); W silica-magnetic nanoparticles aminosilanised in water and T aminosilanised silica-magnetite nanoparticles produced via TPRE; 1 and 2 correspond to 16S rRNA and Salmonella enterica DNA sequences, respectively; C+, positive PCR control.