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. 2012 Apr 3;40(14):e105. doi: 10.1093/nar/gks285

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Pentaprobe nucleic acid binding by the zinc fingers of ZRANB2. GST, GST-F1, GST-F2 and GST-F12 (∼15 μM) were incubated with ³²P-labeled Pentaprobe in the form of (A) dsDNA and (B) ssRNA. All complexes were analysed on 6% polyacrylamide native gels. (A) ssDNA mixed with: Lane 1: buffer; Lane 2: GATA 1 C-terminal finger; Lane 3: GST; Lane 4: ZRANB2 GST-F12. (B) ssRNA mixed with: Lane 1: buffer; Lane 2: GST; Lane 3: ZRANB2 GST-F1; Lane 4: ZRANB2 GST-F2; Lane 5 and 6: decreasing concentrations of ZRANB2 GST-F12; Lane 7: ZRANB2 GST-F12 in complex with a monoclonal anti-GST antibody; Lane 8: buffer.