Skip to main content
. 2012 Apr 3;40(14):e105. doi: 10.1093/nar/gks285

Figure 7.

Figure 7.

The effect of heparin on Pentaprobe EMSAs. EMSAs showing that the binding of GST-ZRANB2 F12 to ssDNA and ssRNA can be modulated by the addition of heparin. (A) ssDNA Pentaprobe mixed with: Lane 1: buffer; Lane 2–6: increasing amounts of GST-ZRANB2 F1232D (1, 2, 4, 8 and 16 µM); Lane 7: buffer; Lane 8–11: 15 µM GST-ZRANB2 F1232D in the presence of 0, 2.3, 23 and 230 µg ml−1 heparin. (B) Internally 32P labeled ssRNA Pentaprobe mixed with: Lane 1–2: buffer; Lane 3–6: increasing amounts of GST-ZRANB2 F1232D (1.2, 3.5, 11 and 22 µM); Lane 7–9: 11 µM GST-ZRANB2 F1232D in the presence of 2.3, 23 and 230 µg ml−1 heparin. All samples were separated on a 6% polyacrylamide gel.