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. 2012 Apr 9;40(14):6922–6935. doi: 10.1093/nar/gks289

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Determination of the salt correction parameter m. Experimental evaluation of the sequence-independent correction parameter m from g₁([Mon⁺]) = m log([Mon⁺]/1000) using Equation 10. Estimators of obtained experimentally (Equation 10) were compared with the expected (Equation 7) profiles for different values of m (0, 0.1, 0.2, 0.3, 0.4 and 0.5 from top to bottom). Red (green) points are the experimental estimators at a pulling rate of 12.5 (1.8) pN/s. Blue (magenta) points are the experimental estimators of at a pulling rate of 12.5 (1.8) pN/s. Light blue lines are the profiles for values of m not matched, and black lines are the experimental estimators of B_eff(f) that match with experiments. Application of the method to experiments done at 1050 mM [Mon⁺] (A), 550 mM [Mon⁺] (B), 150 mM [Mon⁺] (C), and 50 mM [Mon⁺] (D).

Inline graphic — Determination of the salt correction parameter m. Experimental evaluation of the sequence-independent correction parameter m from g₁([Mon⁺]) = m log([Mon⁺]/1000) using Equation 10. Estimators of obtained experimentally (Equation 10) were compared with the expected (Equation 7) profiles for different values of m (0, 0.1, 0.2, 0.3, 0.4 and 0.5 from top to bottom). Red (green) points are the experimental estimators at a pulling rate of 12.5 (1.8) pN/s. Blue (magenta) points are the experimental estimators of at a pulling rate of 12.5 (1.8) pN/s. Light blue lines are the profiles for values of m not matched, and black lines are the experimental estimators of B_eff(f) that match with experiments. Application of the method to experiments done at 1050 mM [Mon⁺] (A), 550 mM [Mon⁺] (B), 150 mM [Mon⁺] (C), and 50 mM [Mon⁺] (D).