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. 2012 Apr 9;40(14):6922–6935. doi: 10.1093/nar/gks289

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Barrier location and mechanical fragility at 50 mM and 1050 mM [Mon⁺]. (A) Force-dependence of the barrier position measured with respect to the F state, . Continuous gray line is the WLC prediction of the molecular extension when n = 19 or n = 6 bps are unzipped at 50 mM [Mon⁺], and dashed gray line corresponds to the WLC prediction when n = 19 or n = 6 bps are unzipped at 1050 mM [Mon⁺]. As seen, at an intermediate value of forces n = 6 coincides with the TS for both ionic conditions. (B) Dependence of fragility μ(f) with force. Gray lines indicate the value of the fragility for different locations n of the TS along the stem. Continuous black lines are the theoretical prediction using Kramers rate theory for data at 50 mM [Mon⁺], and dashed black lines for data at 1050 mM [Mon⁺]. Blue and green points are the experimental evaluation of and μ(f) for folding and unfolding data collected at 50 mM [Mon⁺]. Red and purple points are the experimental evaluation for folding and unfolding at 1050 mM [Mon⁺].

Inline graphic — Barrier location and mechanical fragility at 50 mM and 1050 mM [Mon⁺]. (A) Force-dependence of the barrier position measured with respect to the F state, . Continuous gray line is the WLC prediction of the molecular extension when n = 19 or n = 6 bps are unzipped at 50 mM [Mon⁺], and dashed gray line corresponds to the WLC prediction when n = 19 or n = 6 bps are unzipped at 1050 mM [Mon⁺]. As seen, at an intermediate value of forces n = 6 coincides with the TS for both ionic conditions. (B) Dependence of fragility μ(f) with force. Gray lines indicate the value of the fragility for different locations n of the TS along the stem. Continuous black lines are the theoretical prediction using Kramers rate theory for data at 50 mM [Mon⁺], and dashed black lines for data at 1050 mM [Mon⁺]. Blue and green points are the experimental evaluation of and μ(f) for folding and unfolding data collected at 50 mM [Mon⁺]. Red and purple points are the experimental evaluation for folding and unfolding at 1050 mM [Mon⁺].