Figure 3.

Binding of specific (A, C, E and G), mis-cognate (B, D, F and H), and non-cognate (I) DNA oligoduplexes by the wt Bse634I. Labels shown on the picture indicate oligoduplex (Table 2) used in the gel-shift experiment. I stands for Inosine and U for Uridine. Concentration of Bse634I in samples is increasing from 0.1 nM to 5 μM (in terms of monomer; see ‘Materials and Methods’ section). The first lane in all gels contains no protein. Specific or specific-like Bse634I–DNA complexes are designated by arrows.