Table 2.

DNA oligoduplexes used in this study

Name	Sequencea	Comment
NS	5′-AGCGTAGCACTGGGCTGCTAGTC-3′	Non-cognate, unmodified
	3′-TCGCATCGTGACCCGACGATCAG-5′
AT	5′-CGCACGATCACCGGTGATGCACGC-3′	Cognate, unmodified
	3′-GCGTGCTAGTGGCCACTACGTGCG-5′
GC	5′-CGCACGATCGCCGGCGATGCACGC-3′	Cognate, unmodified
	3′-GCGTGCTAGCGGCCGCTACGTGCG-5′
TA	5′-CGCACGATCTCCGGAGATGCACGC-3′	Mis-cognate, unmodified
	3′-GCGTGCTAGAGGCCTCTACGTGCG-5′
CG	5′-CGCACGATCCCCGGGGATGCACGC-3′	Mis-cognate, unmodified
	3′-GCGTGCTAGGGGCCCCTACGTGCG-5′
AU	5′-CGCACGATCACCGGUGATGCACGC-3′	Cognate, modified
	3′-GCGTGCTAGUGGCCACTACGTGCG-5′
IC	5′-CGCACGATCICCGGCGATGCACGC-3′	Cognate, modified
	3′-GCGTGCTAGCGGCCICTACGTGCG-5′
UA	5′-CGCACGATCUCCGGAGATGCACGC-3′	Mis-cognate, modified
	3′-GCGTGCTAGAGGCCUCTACGTGCG-5′
CI	5′-CGCACGATCCCCGGIGATGCACGC-3′	Mis-cognate, modified
	3′-GCGTGCTAGIGGCCCCTACGTGCG-5′
GC-1	5′-TCGCGCCGGCGCG-3′	Crystallization
	3′-GCGCGGCCGCGCT-5′
AT-1	5′-TCGCACCGGTGCG-3′	Crystallization
	3′-GCGTGGCCACGCT-5′
AT-2	5′-TTCGACCGGTCGA-3′	Crystallization
	3′-AGCTGGCCAGCTT-5′

^aThe central tetranucleotide CCGG of the Bse634I recognition sequence is underlined. Modified bases are in bold face.