Table 3.

Rate constants of oligoduplex cleavage by Bse634I

Oligoduplex	k (min−1)
	wt		P203S		P203G
	25°C	50°C	25°C	50°C	25°C	50°C
AT	10 ± 1.3	–	15 ± 1.8	–	2.9 ± 0.2	–
GC	4.1 ± 0.9	–	7.8 ± 1.2	–	12 ± 4.0	–
AU	5.4 ± 1.3	–	11 ± 2.4	–	1.5 ± 0.4	–
IC	13 ± 2.9	–	17 ± 1.5	–	14 ± 2.1	–
TA	nca	5.7 ± 0.7 × 10−5	nc	5.7 ± 1.6 × 10−4	nc	1.2 ± 0.3 × 10−4
CG	nc	8.2 ± 0.8 × 10−5	1.3 ± 0.1 × 10−4	9.4 ± 1 × 10−2	nc	3.9 ± 0.5 × 10−4
UA	nc	6.9 ± 0.8 × 10−2	1.1 ± 0.1 × 10−2	5.8 ± 1	1.7 ± 2.6 × 10−3	3.8 ± 0.3 × 10−1
CI	nc	3.8 ± 0.9 × 10−5	1.4 ± 0.1 × 10−4	2.4 ± 0.8 × 10−1	nc	1.5 ± 0.9 × 10−3
NS	nc	nc	nc	5.4 ± 1.6 × 10−5	nc	3.7 ± 2 × 10−5

^aNo cleavage products were observed after 48 h of incubation.