Figure 1.

Induction of miR-145 by resveratrol (RSV). (A) Expression of p53 in MCF-10A, 231 and BT-549, MCF-7 and T47D by western. (B) RSV at 30 µM induced miR-145 in MDA-MB-231 and BT-549 cells, respectively. However, in MCF-7 cells the same concentration of RSV did not induce miR-145 until the concentration was increased to 100 µM. Cells were treated with RSV for 24 h before RNA extraction, followed by real time RT–PCR. (C) In situ hybridization also detected an increase of miR-145 after RSV treatment (30 µM) in MDA-MB-231 cells. Values in (B) are mean ± SE, n = 3. **P < 0.01 compared with vector control.