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. 2012 Apr 20;40(14):e110. doi: 10.1093/nar/gks326

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — (A) A transient of an immobilized origami-bound TATA–oligo visualizes the rapid binding and unbinding of TBP to the TATA–box (at 35°C, 1 µM TBP). At 40 s the acceptor ATTO647N bleaches and the donor fluorophore Cy3B shows increased fluorescence. (B) The TBP-induced bending is shown in high-time resolution of 20 ms/frame (Seconds 2–5 of panel A). (C and D). FRET efficiency over time is changing between 0.19 (low FRET of straight DNA) and 0.47 (TBP induced high FRET due to DNA bending).