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. 2012 May 8;40(14):6547–6557. doi: 10.1093/nar/gks346

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Effects of RbpA on σ^A and σ^F dependent transcription. (A and B): Sequences of the rrnAP3 and usfXP1 promoters. The −10 and −35 consensus elements are underlined, and transcription start sites (TSS) are marked by arrows. (C and D) Abortive transcription carried out by the σ^A-containing RNAP (Eσ^A) on the rrnAP3 template and σ^F-containing RNAP (Eσ^F) on the usfXP1 template. Lanes 2–5: RbpA was added to 0.15, 0.3, 0.6 and 1.2 µM, respectively. Quantification of the bands indicated by arrows is shown on the bottom graphs. The RNA amounts were normalized to the value obtained without RbpA (lane 1). The error bars are the SD of triplicate experiments. (E and F) Kinetics of single-round run-off transcription carried out by σ^A-containing RNAP on the rrnAP3 and by σ^F-containing RNAP on the usfXP1 template, respectively. RbpA (1.2 μM) was added to the reaction when indicated. The quantification of the run-off RNA is shown on the bottom graphs. The RNA amounts were normalized to the amount of RNA synthesized without RbpA after 10 min of transcription (rrnAP3) or 5 min of transcription (usfXP1). The error bars are the SD of duplicate experiments.