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. 2012 May 8;40(14):6547–6557. doi: 10.1093/nar/gks346

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Influence of RbpA on RNAP inhibition by rifampicin. (A) Abortive transcription initiation from the rrnAP3 promoter by the σ^A-containing RNAP in the presence of [³²P]-UTP, ATP and GTP. Rifampicin (Rif) was added to the reactions at the indicated concentrations. RbpA (1.2 μM) was added to the reactions when indicated. (B) Quantification of the experiment shown in (A). The amounts of abortive RNA synthesized in the presence of different concentrations of rifampicin were normalized to the amount of the RNA synthesized without rifampicin (the first lane) and plotted against rifampicin concentrations. The error bars are the SD of duplicate experiments.