Upstream (5′–3′) |
|
|
U1 |
26 |
CGCCAGGGTTTTCCCAGTCACGACCA |
U2 |
26 |
CGACCGTGCCAGCCTAAATTTCAATT |
U3 |
20 |
CGACCGTGCCAGCCTAAATT |
U4 |
23 |
CGACCGTGCCAGCCTAAATTTCA |
Downstream (5′–3′) |
|
|
D1.2 |
25 |
GTCGTTTTACAACGACGTGACTGGG |
D1.5 |
28 |
GCCGTCGTTTTACAACGACGTGACTGGG |
D1.10 |
33 |
CACTGGCCGTCGTTTTACAACGACGTGACTGGG |
D1.15 |
38 |
TAATTCACTGGCCGTCGTTTTACAACGACGTGACTGGG |
D1.20 |
43 |
TTAGTTAATTCACTGGCCGTCGTTTTACAACGACGTGACTGGG |
D1.30 |
53 |
TTCACGCCTGTTAGTTAATTCACTGGCCGTCGTTTTACAACGACGTGACTGGG |
D2.15 |
41 |
ATACGCGCTATAACCCCCGTCCACCCGACGCCACCTCCTGC |
D2.30 |
56 |
TTCTACTTCCAATTGATACGCGCTATAACCCCCGTCCACCCGACGCCACCTCCTGC |
Template (3′–5′) |
|
|
T1 |
49 |
GCGGTCCCAAAAGGGTCAGTGCTGGGCAAAATGTTGCTGCACTGACCCG |
T2 |
51 |
GCTGGCACGGTCGGATTTAAAGTTAGGGCAGGTGGGCTGCGGTGGAGGACG |
T3 |
26 |
GGGCAGGTGGGCTGCGGTGGAGGACG |