Table 1.
Oligonucleotide sequences
Oligo | Length (nt) | Sequence |
---|---|---|
Upstream (5′–3′) | ||
U1 | 26 | CGCCAGGGTTTTCCCAGTCACGACCA |
U2 | 26 | CGACCGTGCCAGCCTAAATTTCAATT |
U3 | 20 | CGACCGTGCCAGCCTAAATT |
U4 | 23 | CGACCGTGCCAGCCTAAATTTCA |
Downstream (5′–3′) | ||
D1.2 | 25 | GTCGTTTTACAACGACGTGACTGGG |
D1.5 | 28 | GCCGTCGTTTTACAACGACGTGACTGGG |
D1.10 | 33 | CACTGGCCGTCGTTTTACAACGACGTGACTGGG |
D1.15 | 38 | TAATTCACTGGCCGTCGTTTTACAACGACGTGACTGGG |
D1.20 | 43 | TTAGTTAATTCACTGGCCGTCGTTTTACAACGACGTGACTGGG |
D1.30 | 53 | TTCACGCCTGTTAGTTAATTCACTGGCCGTCGTTTTACAACGACGTGACTGGG |
D2.15 | 41 | ATACGCGCTATAACCCCCGTCCACCCGACGCCACCTCCTGC |
D2.30 | 56 | TTCTACTTCCAATTGATACGCGCTATAACCCCCGTCCACCCGACGCCACCTCCTGC |
Template (3′–5′) | ||
T1 | 49 | GCGGTCCCAAAAGGGTCAGTGCTGGGCAAAATGTTGCTGCACTGACCCG |
T2 | 51 | GCTGGCACGGTCGGATTTAAAGTTAGGGCAGGTGGGCTGCGGTGGAGGACG |
T3 | 26 | GGGCAGGTGGGCTGCGGTGGAGGACG |