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. 2012 May 8;40(14):6774–6786. doi: 10.1093/nar/gks388

Table 1.

Oligonucleotide sequences

Oligo Length (nt) Sequence
Upstream (5′–3′)
    U1 26 CGCCAGGGTTTTCCCAGTCACGACCA
    U2 26 CGACCGTGCCAGCCTAAATTTCAATT
    U3 20 CGACCGTGCCAGCCTAAATT
    U4 23 CGACCGTGCCAGCCTAAATTTCA
Downstream (5′–3′)
    D1.2 25 GTCGTTTTACAACGACGTGACTGGG
    D1.5 28 GCCGTCGTTTTACAACGACGTGACTGGG
    D1.10 33 CACTGGCCGTCGTTTTACAACGACGTGACTGGG
    D1.15 38 TAATTCACTGGCCGTCGTTTTACAACGACGTGACTGGG
    D1.20 43 TTAGTTAATTCACTGGCCGTCGTTTTACAACGACGTGACTGGG
    D1.30 53 TTCACGCCTGTTAGTTAATTCACTGGCCGTCGTTTTACAACGACGTGACTGGG
    D2.15 41 ATACGCGCTATAACCCCCGTCCACCCGACGCCACCTCCTGC
    D2.30 56 TTCTACTTCCAATTGATACGCGCTATAACCCCCGTCCACCCGACGCCACCTCCTGC
Template (3′–5′)
    T1 49 GCGGTCCCAAAAGGGTCAGTGCTGGGCAAAATGTTGCTGCACTGACCCG
    T2 51 GCTGGCACGGTCGGATTTAAAGTTAGGGCAGGTGGGCTGCGGTGGAGGACG
    T3 26 GGGCAGGTGGGCTGCGGTGGAGGACG