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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Mol Cancer Ther. 2012 Apr 5;11(6):1236–1246. doi: 10.1158/1535-7163.MCT-11-0936

Figure 3. PDK1 contributes to EGFR-independent p70S6K phosphorylation.

Figure 3

(A) HNSCC (1483) cells were transfected with NTC, EGFR, PDK1 or a combination of EGFR and PDK1 siRNA for 72 hours. Lysates collected were analyzed for phospho-p70S6K, p70S6K, PDK1 and β-tubulin. The results are representative of 3 independent experiments. (B) 1483 cells were stably transfected with pcDNA3 (VC) or pcDNA3-PDKM (kinase-dead). 1483 VC and PDKM cells were transfected with NTC or EGFR siRNA for 72 hours. Lysates were collected and resolved by SDS-PAGE. The results are representative of 2 experiments.