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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: Nitric Oxide. 2011 Dec 17;26(2):81–88. doi: 10.1016/j.niox.2011.12.004

Table 1.

Experimental design indicating the sequence of stimuli delivered to individual mice over a two week study period.*

Mouse Week 1
Sessions		 Week 2
Sessions
Monday Wednesday Friday Monday Wednesday Friday
1 2000 Hz, 250 Hz, 5 Hz 250 Hz, 5 Hz, 2000 Hz 5 Hz, 2000 Hz, 250 Hz 250 Hz, 2000 Hz, 5 Hz 5 Hz, 250 Hz, 2000 Hz 2000 Hz, 5 Hz, 250 Hz
2 250 Hz, 5 Hz, 2000 Hz 5 Hz, 2000 Hz, 250 Hz 5 Hz, 250 Hz, 2000 Hz 2000 Hz, 5 Hz, 250 Hz 250 Hz, 2000 Hz, 5 Hz 2000 Hz, 250 Hz, 5 Hz
3 5 Hz, 2000 Hz, 250 Hz 2000 Hz, 250 Hz, 5 Hz 2000 Hz, 5 Hz, 250 Hz 5 Hz, 250 Hz, 2000 Hz 250 Hz, 5 Hz, 2000 Hz 250 Hz, 2000 Hz, 5 Hz
4 250 Hz, 2000 Hz, 5 Hz 5 Hz, 250 Hz, 2000 Hz 2000 Hz, 250 Hz, 5 Hz 250 Hz, 5 Hz, 2000 Hz 2000 Hz, 5 Hz, 250 Hz 5 Hz, 2000 Hz, 250 Hz
5 5 Hz, 250 Hz, 2000 Hz 2000 Hz, 5 Hz, 250 Hz 250 Hz, 2000 Hz, 5 Hz 5 Hz, 2000 Hz, 250 Hz 2000 Hz, 250 Hz, 5 Hz 250 Hz, 5 Hz, 2000 Hz
6 2000 Hz, 5 Hz, 250 Hz 250 Hz, 2000 Hz, 5 Hz 250 Hz, 5 Hz, 2000 Hz 2000 Hz, 250 Hz, 5 Hz 5 Hz, 2000 Hz, 250 Hz 5 Hz, 250 Hz, 2000 Hz
*

Animals were studied in groups of six (a total of six groups of six animals). The assignment of their random order of electrical stimulus was maintained for each group of animals. Measurements of current vocalization threshold were obtained over two-weeks.