Table 1. Formulations of 33 crystallization solutions (28 unique) that failed to dispense from a total of 480 from the JCSG+, Classics, Index, Wizards and PEG MW screens.
The ten paired duplicates in this list are marked in bold. 26 of these solutions (21 unique) contained MPD (2-methyl-2,4-pentanediol). JCSG+ No. 79, Classics No. 45 and Wizards No. 88 are marked with an asterisk as these conditions were transferred measurably in another screen. nm, not measured.
| Solution No. | Formulation | Viscosity (mPa s) | Density (g cm−3) |
|---|---|---|---|
| JCSG+ 41 (137) | 70%(v/v) MPD, 0.1 M HEPES pH 7.5 | 21.3 | 0.98 |
| JCSG+ 11 (107) | 50%(v/v) MPD, 0.2 M ammonium phosphate, 0.1 M Tris pH 8.5 | 10.2 | 1.01 |
| JCSG+ 88 (184) | 45%(v/v) MPD, 0.2 M calcium chloride, 0.1 M bis-tris pH 5.5 | 8.2 | 1.02 |
| JCSG+ 89 (185) | 45%(v/v) MPD, 0.2 M ammonium acetate, 0.1 M bis-tris pH 5.5 | 7.7 | 1 |
| JCSG+ 96 (192) | 45%(v/v) MPD, 0.2 M ammonium acetate, 0.1 M HEPES pH 7.5 | 7.8 | 1 |
| JCSG+ 17 (113) | 40%(v/v) MPD, 0.1 M cacodylate pH 6.5 | 14.8 | 1.01 |
| JCSG+ 44 (140) | 40%(v/v) MPD, 0.1 M Tris pH 8 | 5.6 | 1 |
| JCSG+ 53 (149) | 40%(v/v) MPD, 0.1 M CAPS pH 10.5 | 6.1 | 1 |
| JCSG+ 61 (157) | 30%(w/v) Jeffamine M-600, 0.05 M cesium chloride, 0.1 M MES pH 6.5 | 5.9 | 1.03 |
| JCSG+ 37 (133) | 24%(w/v) PEG 1500, 20%(v/v) glycerol | 11.04 | 1.1 |
| JCSG+ 79 (175)* | 15%(w/v) PEG 3350, 0.1 M succinic acid pH 7.0* | 4.83 | 1.03 |
| Classics 22 (22) | 70%(v/v) MPD, 0.1 M HEPES pH 7.5 | 19 | 0.98 |
| Classics 21 (21) | 50%(v/v) MPD, 0.2 M ammonium phosphate, 0.1 M Tris pH 8.5 | 9.8 | 1 |
| Classics 17 (17) | 30%(v/v) MPD, 0.2 M ammonium acetate, 0.1 M sodium citrate pH 5.6 | 4.4 | 1.02 |
| Classics 18 (18) | 30%(v/v) MPD, 0.2 M magnesium acetate, 0.1 M cacodylate pH 6.5 | 5 | 1.02 |
| Classics 19 (19) | 30%(v/v) MPD, 0.2 M sodium citrate, 0.1 M HEPES pH 7.5 | 5.3 | 1.04 |
| Classics 20 (20) | 30%(v/v) MPD, 0.5 M ammonium sulfate, 0.1 M HEPES pH 7.5 | 4.8 | 1.04 |
| Classics 45 (45)* | 1.6 M trisodium citrate* | 7.3 | 1.25 |
| Index 48 | 45%(v/v) MPD, 0.2 M calcium chloride dihydrate, 0.1 M bis-tris pH 5.5 | nm | nm |
| Index 49 | 45%(v/v) MPD, 0.2 M calcium chloride dihydrate, 0.1 M bis-tris pH 6.5 | nm | nm |
| Index 50 | 45%(v/v) MPD, 0.2 M ammonium acetate, 0.1 M bis-tris pH 5.5 | nm | nm |
| Index 51 | 45%(v/v) MPD, 0.2 M ammonium acetate, 0.1 M bis-tris pH 6.5 | nm | nm |
| Index 52 | 45%(v/v) MPD, 0.2 M ammonium acetate, 0.1 M HEPES pH 7.5 | nm | nm |
| Index 53 | 45%(v/v) MPD, 0.2 M ammonium acetate, 0.1 M Tris pH 8.5 | nm | nm |
| Index 58 | 45%(v/v) polypropylene glycol P 400, 0.1 M bis-tris pH 6.5 | nm | nm |
| Index 56 | 35%(v/v) pentaerythritol propoxylate, 0.2 M potassium chloride, 0.05 M HEPES pH 7.5 | nm | nm |
| Wizards 4 | 35%(v/v) MPD, 0.2 M magnesium chloride, 0.1 M imidazole pH 8.0 | nm | nm |
| Wizards 24 | 35%(v/v) MPD, 0.2 M sodium chloride, 0.1 M Tris pH 7.0 | nm | nm |
| Wizards 43 | 35%(v/v) MPD, 0.1 M sodium/potassium phosphate pH 6.2 | nm | nm |
| Wizards 50 | 35%(v/v) MPD, 0.2 M lithium sulfate, 0.1 M MES pH 6.0 | nm | nm |
| Wizards 69 | 35%(v/v) MPD, 0.1 M sodium acetate pH 4.5 | nm | nm |
| Wizards 73 | 35%(v/v) MPD, 0.2 M sodium chloride, 0.1 M HEPES pH 7.5 | nm | nm |
| Wizards 88* | 20%(w/v) PEG 3000, 0.2 M zinc acetate, 0.1 M imidazole pH 8.0* | nm | nm |