Table 2. Kinetic data for wild-type and C141S CeGNA1.

The apparent K _m and k _cat values for acetyl-CoA and GlcN-6P and the catalytic efficiency were determined from the amounts of CoA produced. n.d., no detectable activity.

	K m (µM)	k cat (s−1)	k cat/K m (s−1 µM −1)
GlcN-6P (acetyl-CoA constant)
WT	125 ± 9	139	0.6
C141S	216 ± 10	28	0.1
Cys–CoA	n.d.	n.d.	n.d.
Acetyl-CoA (GlcN-6P constant)
WT	192 ± 16	252	1.3
C141S	261 ± 33	48	0.2
Cys–CoA	n.d.	n.d.	n.d.