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. 2012 Sep;53(9):1877–1889. doi: 10.1194/jlr.M026591

Fig. 6.

Fig. 6.

Cideb−/− hepatocytes have smaller and more clustered LDs, and the knockdown of perilipin 2 increases the size of LDs. (A) Fluorescent microscopic images of hepatocytes isolated from wild-type or Cideb–/– mice and stained with Bodipy 493/503 (green). Scale bars, 10 μm. (B) EM images of hepatocytes isolated from wild-type and Cideb–/– mice. Scale bars, 10 μm. (C–F) Knockdown of perilipin 2 increases the LD size in wild-type (C, D) and Cideb-/- (E, F) hepatocytes. Wild-type and Cideb–/– mice were injected with adenoviruses containing Ad-shPerilipin 2 or Ad-ctrl shRNA (n = 5 each) for a week. Hepatocytes were isolated and incubated with 400 μM OA for 12 h before staining. The LDs were stained with Bodipy 493/503 (green). Fluorescent microscope images (C, E) and statistical analysis (D, F) showing the LD size distribution. Scale bars, 5 μm. *P < 0.05, **P < 0.01, ***P < 0.001. Data are the means ± SEM.