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TSA treatment promotes p-ERK1/2 detachment from the CYP46A1 promoter. ChIP was performed using chromatin prepared from SH-SY5Y control cells and cells treated with 250 nM TSA for the indicated time points. After chromatin precipitation with an anti-p-ERK1/2 antibody, the recovered DNA was evaluated by real-time PCR. Results represent means ± SEM of at least three independent experiments and are expressed as a percentage of total input (*P < 0.05).
