Fig. 7.

The ESCRT pathway and PCSK9-mediated LDLR degradation in HEK293 cells. A: HEK293 cells were cultured on cover slides as indicated in Materials and Methods, and siRNA transfection was performed as described in Fig. 1. After 72 h, cells were fixed and stained with anti-EEA1 antibody as indicated in Materials and Methods. B: HEK293 cells were cultured, and siRNA transfection was performed as described in Fig. 1. After 72 h, cells were treated with PCSK9 (10 μg/ml) for the indicated times. Cell lysates were subjected to immunoblot analysis. Graphs represent the quantitative results from this experiment. The experiments were performed twice with similar results. Graphs represent the data shown in the experiment.