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Characterization of purified human recombinant Trx-tagged apolipoprotein O (Trx-apoO; A) and monoclonal antibody (MAb; B). A: Purified Trx-apoO (1µg) was analyzed by SDS-PAGE and visualized by Coomassie brilliant blue. B: Human plasma (1µl; lane 1), culture medium of HepG2 cells (1µl; lane 2), and purified bacterial Trx-apoO (0.2 ng; lane 3) were subjected to SDS-PAGE under reducing conditions. Immunoblotting with MAb was performed as described in Methods.
