Fig. 5.

Reaction progress curves of bee venom PLA₂ catalyzed hydrolysis of 100 μM DPPC and DOPC bilayers. GP of ADIFAB fluorescence in SUV and MLV of (A) DPPC and (B) DOPC. Two microliters of enzyme solution at a concentration of 0.04 mg/ml was added to the sample solution of volume 2 ml. The solid lines are linear fits to the initial linear region. The slopes of these lines yield the initial rate of hydrolysis. C: product formed measured by pH-Stat in the DPPC and DOPC bilayers. Three microliters of enzyme solution at a concentration of 0.8 mg/ml was added to the sample solution of volume 5 ml.