TABLE I.
Summary of results of fitting fluorescence recovery curves in the presence of reflective boundaries to diffusion with the standard MP-FRAP diffusive-recovery model. Boundaries are located symmetrically about the focal volume center at positions measured in units of ωr or ωz. The boundary locations for a single parallel-plane boundary and two parallel-plane boundaries laying parallel to the optical axis within which the standard MP-FRAP model yields erroneous diffusion coefficients can be narrowed by using the new models presented in the text.
| Boundary geometry | Orientation with respect to optical axis | Boundary limit with standard MP-FRAP | Boundary limit with new model |
|---|---|---|---|
| Single plane | parallel | 1.3ωr–1.5ωr | ~–0.15ωr |
| perpendicular | ~–0.3ωz | ||
| Parallel-plane | parallel | 1.5ωr–1.8ωr | 1ωr |
| perpendicular | <0.1ωz–~0.5ωz | ||
| Cylinder | parallel | 1.8ωr–2ωr | |
| perpendicular | 0.3ωz –0.7ωz | ||
| Sphere | 2.75ωr –3.75ωr |