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. Author manuscript; available in PMC: 2012 Aug 7.
Published in final edited form as: Curr Pharm Biotechnol. 2010 Aug;11(5):518–526. doi: 10.2174/138920110791591463

Fig. (2). Knockdown of Ago2 inhibits RNAa and RNAi function.

Fig. (2)

(A) PC-3 cells were transfected at 50 nM siCon or dsEcad-215 for 72 hours. Combination treatments of dsEcad-215 and Ago-specific siRNAs (siAgo1, 2, 3, or 4) were performed using 25 nM concentrations of each RNA duplex. E-cadherin, Ago1-4, and GAPDH expression levels were assessed by standard RT-PCR. GAPDH served as a loading control. (B) PC-3 cells were transfected at 50 nM siCon or siMOF for 72 hours. Co-treatments of siMOF with siAgo1-4 were performed at 25 nM each siRNA. mRNA expression levels were assessed by standard RT-PCR.