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. Author manuscript; available in PMC: 2012 Aug 7.
Published in final edited form as: Curr Pharm Biotechnol. 2010 Aug;11(5):518–526. doi: 10.2174/138920110791591463

Fig. (3). Strand modifications that manipulate RNAa activity.

Fig. (3)

(A) Schematic representation of modified saRNA covalently linked to biotin at the 5’-end of the antisense (dsRNA-AS-5’Bio) or sense (dsRNA-S-5’Bio) strand. Unmodified saRNA (dsRNA) is also depicted. The antisense strand in each duplex is red, while the sense strand is in black. (B-C) PC-3 cells were transfected at 50 nM concentrations of the indicated saRNAs for 72 hours. Mock samples were transfected in the absence of saRNA. Expression levels of E-cadherin (B) or p21 (C) were assessed by standard RT-PCR. GAPDH levels were also evaluated to serve as loading controls. (D) Schematic representation of modified saRNA possessing a mismatched base opposite the 5’ most nucleotide of either the antisense (dsRNA-AS-MM) or sense (dsRNA-S-MM) strand. (E-F) PC-3 cells were transfected with the indicated saRNAs for 72 hours. Expression levels of GAPDH and E-cadherin (E) or p21 (F) were assessed by standard RT-PCR. (G) Schematic depiction of dsP21-322-AS-5’Bio-S-MM. (H) PC-3 cells were transfected with the indicated saRNAs for 72 hours. Expression of p21 and GAPDH were evaluated by standard RT-PCR.