Figure 5. iBCSCs overexpress Oct4, Sox2, Nanog, and Klf4, but not c-Myc.
SUM159PT-ZsGreen-ODC cells were sorted into ZsGreen-cODC-positive and -negative cells. ZsGreen-cODC-negative cells were plated as monolayers, and irradiated the following day with 4 or 8 Gy. iBCSCs cells were sorted at day 5 post-irradiation. Expression of Oct4, Sox2, Nanog, Klf4, and c-Myc was analyzed by semi-quantitative RT-PCR. The means of transcription factor gene expression levels (n=4) are shown, * indicates p<0.05.