Table 1.

Quantification of the colocalization of DDX6 with Gag in HIV-1–infected cells

	Experiment 1		Experiment 2		Experiment 1 and 2 combined		Fold colocalization
	WT Gag	GagΔp2-NC-p1-p6	WT Gag	GagΔp2-NC-p1-p6	WT Gag	GagΔp2-NC-p1-p6	WT Gag	GagΔp2-NC-p1-p6
Membrane length (µm)	111	150	155	166	266	316
Total DDX6 label	643	536	624	679	1,267	1,215
Total targeted Gag clusters	67	499	67	379	134	878
Targeted Gag clusters with DDX6	22	165	19	108	41	273	1.00	(1.00)
Gag clusters at early assembly sites	58	0	56	0	114	0
Gag clusters at early assembly sites with DDX6	41	n/a	36	n/a	77	n/a	2.20a
Gag clusters at late assembly sites	20	0	57	0	77	0
Gag clusters at late assembly sites with DDX6	17	n/a	46	n/a	63	n/a	2.70a

Cells infected with the indicated proviruses were double immunogold labeled for Gag and DDX6. In two independent labeling experiments, the following parameters were quantified: the total length of membrane examined, the total number of independent DDX6 labeling events within 100 nm of the membrane, the total number of independent targeted, early, and late Gag clusters, and the total number of Gag clusters that were associated with DDX6 classified by stage of assembly (i.e., targeted Gag or Gag at early or late assembly sites). Fold colocalization is defined as the number of WT Gag clusters colocalized with DDX6 for each stage of assembly, normalized to Gag-DDX6 colocalization in the GagΔp2-NC-p1-p6 negative control (indicated by parentheses). Fold colocalization (bold) was calculated for each experiment. The fold colocalization averaged from the two experiments is shown and is also graphed with SD in Fig. 5.

^aP < 0.05.