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. 2012 Aug 6;198(3):405–420. doi: 10.1083/jcb.201202097

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© 2012 Moreira et al.

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Figure 6. — Seg1 rods form without Pil1 and contain eisosome components. (A) Projections from confocal stacks of wild-type (WT), pil1Δ, and pil1Δ lsp1Δ cells lacking endogenous Seg1 and expressing Seg1-GFP from the URA3 locus. (B) Projections of WT cells expressing Pil1-cherry, lacking endogenous Seg1, and expressing Seg1-GFP from the URA3 locus. (C) Projections of pil1Δ cells expressing Lsp1-cherry, lacking endogenous Seg1, and expressing Seg1-GFP from the URA3 locus. (D) Epifluorescence images of WT cells lacking endogenous Seg1, expressing Seg1-GFP from the URA3 locus, and stained with filipin to visualize ergosterol. Arrows indicate colocalization of Seg1-GFP and filipin. Bars, 5 µm.