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. 2012 Aug 6;198(3):331–341. doi: 10.1083/jcb.201204086

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© 2012 Bellis et al.

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Figure 4. — Atoh1 and Muc2 doublet sister cell analysis. (green) β-Catenin; (magenta) EdU; (blue) DAPI. (A and A′) Distribution of EdU and Atoh1 (red) in a section grazing the crypt lumen. The marked doublet is Atoh1^+/+. (B) Muc2 (red) and EdU. Muc2⁺ cells contain varying amounts of mucin. The arrow indicates a dividing Muc2⁺ cell. (C–D′′) EdU marked doublets showing one Atoh1⁺ and one Atoh1⁻ sister (see also Videos 8 and 9). In D–D′′, only one of three doublets is shown (marked by stars). The others are visible in Video 9. The data shown are from a single representative experiment out of three repeats. Bars: (A and B) 10 µm; (C and D) 5 µm.