Table 2.
Deletion and stop codon mutants constructed using cosmids or BACs in VZV and their effect on growth in vitro.
| VZV Gene | Mutation | Growth in Vitro | Reference |
|---|---|---|---|
| ORFS/L (ORF0) | del | impaired | Zhang et al. 2007 |
| ORF1 | stop | no change | Cohen and Seidel 1995 |
| del | slight reduced | Zhang et al. 2007 | |
| ORF2 | del | no change | Sato et al. 2002b |
| del | no change | Zhang et al. 2007 | |
| ORF3 | del | slight reduced | Zhang et al. 2007 |
| ORF4 | del | essential | Cohen et al. 2005 |
| del | essential | Sato et al. 2003b | |
| del | essential | Zhang et al. 2007 | |
| ORF5 (gK) | del | essential | Mo et al. 1999 |
| ORF8 | stop | no change | Ross et al. 2007 |
| delete+ | reduced | Ross et al. 2007 | |
| ORF9 | start | essential | Tischer et al. 2007 |
| del | essential | Che et al. 2008 | |
| ORF9A | stop | no change | Ross et al. 1997 |
| ORF10 | del | no change | Cohen and Seidel 1994b |
| del | no change | Che et al. 2008 | |
| ORF11 | del | no change | Che et al. 2008 |
| ORF12 | del | no change | Che et al. 2008 |
| ORF13 | stop | no change | Cohen and Seidel 1993 |
| ORF14 (gC) | stop | no change | Cohen and Seidel 1994a |
| ORF17 | del | reduced | Sato et al. 2002a |
| ORF19 | del | reduced | Heineman and Cohen 1994 |
| ORF21 | del | essential | Xia et al. 2003 |
| ORF23 | del | reduced | Chaudhuri et al. 2008 |
| ORF29 | del | essential | Cohen et al. 2007 |
| ORF32 | del | no change | Reddy et al. 1998a |
| ORF35 | del | reduced | Ito et al. 2005 |
| ORF47 | stop | no change | Heineman and Cohen 1995 |
| ORF49 | del | reduced | Sadaoka et al. 2007 |
| ORF50 | del | reduced | Yamagishi et al. 2008 |
| ORF57 | del | no change | Cox et al. 1998 |
| ORF58 | del | no change | Yoshii et al. 2008 |
| ORF59 | del | no change | Reddy et al. 1998b |
| ORF61 | del | reduced | Cohen and Nguyen 1998 |
| ORF62 | del | essential | Sato et al. 2003a |
| ORF63 | del | essential | Sommer et al. 2001 |
| del | reduced | Cohen et al. 2004 | |
| ORF65 | del | no change | Cohen et al. 2001 |
| del | no change | Niizuma et al. 2003 | |
| ORF66 | stop | no change | Heineman et al. 1996 |
| ORF67 (gI) | del | reduced* or essential | Cohen and Nguyen 1997 |
| del | reduced | Mallory et al. 1997 | |
| ORF68 (gE) | del | essential | Mallory et al. 1997 |
| del | essential | Ali et al. 2009 |
+
interrupts expression of ORF8 and ORF9A
*
reduced in melanoma cells, essential for Vero cells
Stop=stop codons, del=deletion, start=mutations in start codon