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. 2012 Jul 17;3(4):e00127-12. doi: 10.1128/mBio.00127-12

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Copyright © 2012 Absalon et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 2 — A V. cholerae Δbap1ΔrbmAΔrbmC triple mutant does not make a biofilm but can recruit the chitinase activity of RbmA–ChiA-2–FLAG to the cell surface. (A and B) Quantification of biofilms formed by wild-type V. cholerae (WT), an exopolysaccharide mutant (ΔvpsL), and a Δbap1ΔrbmAΔrbmC mutant (triple) carrying an empty vector (pCTL) or a vector encoding RbmA (pRbmA) (A) and the pellicle formed by wild-type V. cholerae (WT) or the Δbap1ΔrbmAΔrbmC triple mutant carrying either an empty vector (pCTL) or plasmids encoding RbmA-FLAG (pRbmA), RbmA-CtxB (pRbmA-CtxB), ChiA-2–FLAG (pChiA-2), or RbmA–ChiA-2–FLAG (pRbmA–ChiA-2) (B). (C and D) Chitinase activity in the cellular fraction (C) and supernatants (D) of V. cholerae Δbap1ΔrbmAΔrbmC mutant carrying an empty vector or a plasmid encoding RbmA-CtxB, ChiA-2–FLAG, or RbmA–ChiA-2–FLAG. Chitinase activity in the cellular fraction of the mutant expressing RbmA–ChiA-2–FLAG was significantly different from those in strains expressing all other recombinant proteins (P < 0.0001). The chitinase activity in the supernatants of mutants expressing ChiA-2–FLAG and RbmA–ChiA-2–FLAG was significantly different from those of the mutant carrying an empty vector (P = 0.034 and P = 0.0172) and the mutant expressing RbmA-CtxB (P = 0.039 and P = 0.0215). The difference in chitinase activity between the supernatants of the strains expressing RbmA–ChiA-2–FLAG and ChiA-2–FLAG was not statistically significant (P = 0.535).