Table 1. Primers for TGFBI amplification.
Exons | Primers | (5′→3′) | Amplicons (bp) |
---|---|---|---|
1 |
BIGH3–1F |
GCTTGCCCGTCGGTCGCTA |
234 |
|
BIGH3–1R |
TCCGAGCCCCGACTACCTGA |
|
2 |
BIGH3–2F |
AGGCAAACACGATGGGAGTCA |
204 |
|
BIGH3–2R |
TAGCACGCAGGTCCCAGACA |
|
3 |
BIGH3–3F |
CCAGATGACCTGTGAGGAACAGTGA |
232 |
|
BIGH3–3R |
CCTTTTATGTGGGTACTCCTCTCT |
|
4 |
BIGH3–4F |
TCCCTCCTTCTGTCTTCTGC |
273 |
|
BIGH3–4R |
AGACTCCCATTCATCATGCC |
|
5 |
BIGH3–5F |
CCTGGGCTCACGAGGGCTGAGAACAT |
387 |
|
BIGH3–5R |
GCCCCTCTTGGGAGGCAATGTGTCCC |
|
6 |
BIGH3–6F |
CCTGGGCTCACGAGGGCTGAGAACAT |
403 |
|
BIGH3–6R |
GCCCCTCTTGGGAGGCAATGTGTCCC |
|
7 |
BIGH3–7F |
GTGAGCTTGGGTTTGGCTTC |
387 |
|
BIGH3–7R |
ACCTCATGGCAGGTGGTATG |
|
8 |
BIGH3–8F |
TGAGGTTATCGTGGAGTG |
435 |
|
BIGH3–8R |
CACATCAGTCTGGTCACA |
|
9 |
BIGH3–9F |
ACTCACGAGATGACATTCCT |
284 |
|
BIGH3–9R |
TCCAGGGACAATCTAACAGG |
|
10 |
BIGH3–10F |
TAGAAGATACCAGATGTTAAGG |
426 |
|
BIGH3–10R |
TGTCAGCAACCAGTTCTCAT |
|
11 |
BIGH3–11F |
GGATAATGACCCTGCTACATGC |
330 |
|
BIGH3–11R |
TCCCCAAGGTAGAAGAAAGC |
|
12 |
BIGH3–12F |
CATTCCAGTGGCCTGGACTCTACTATC |
318 |
|
BIGH3–12R |
GGGGCCCTGAGGGATCACTACTT |
|
13 |
BIGH3–13F |
CCTCCTTGACCAGGCTAATTAC |
299 |
|
BIGH3–13R |
GGCTGCAACTTGAAGGTTGTG |
|
14 |
BIGH3–14F |
CTGTTCAGTAAACACTTGCT |
261 |
|
BIGH3–14R |
CTCTCCACCAACTGCCACAT |
|
15 |
BIGH3–15F |
CCCTCAGTCACGGTTGTT |
348 |
|
BIGH3–15R |
GGAGTTGCCTTGGTTCTT |
|
16 |
BIGH3–16F |
CTTGCACAACTTATGTCTGC |
319 |
|
BIGH3–16R |
TGCACCATGATGTTCTTATC |
|
17 |
BIGH3–17F |
AGTGAAGTTTCACAAACCAC |
475 |
BIGH3–17R | CCACATTTGGGATAGGTC |