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Fluorescent images of HCE cells incubated with same amount of FITC that loaded by CS/TCS-SA NPs for 8 h. The appearance of fluorescence signal of FITC (color of green) inside cells indicated the actual release of FITC from NPs. It showed that the in vitro cell uptake degree of NPs and TCS-SA NPs delivered much more FITC into cells than CS-SA NPs (as the arrows showed). A: CS-SA NPs; B: TCS-SA NPs (Scale bar: 100 μM).
