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. 2012 Jul 18;18:1955–1959.

Table 2. Primers used for mitochondrial haplogrouping.

Primer # Primer sequence Annealing temp. Product size
MIT-1-F
CCTCCCTGTACGAAAGGACA
57
614
MIT-1-R
TGAGATTGTTTGGGCTACTGC
 
 
MIT-2-F
CGAGCAGTAGCCCAAACAAT
57
664
MIT-2-R
TTTTGGATTCTCAGGGATGG
 
 
MIT-3-F
CCATCCCTGAGAATCCAAAA
57
653
MIT-3-R
ATTTTGCGTAGCTGGGTCTG
 
 
MIT-4-F
TAAACCAGACCCAGCTACGC
55
661
MIT-4-R
AAAGTGGCTGATTTGCGTTC
 
 
MIT-5-F
TCAACTGAACGCAAATCAGC
60
784
MIT-5-R
TGAAATTGATGGCCCCTAAG
 
 
MIT-6-F
CTTAGGGGCCATCAATTTCA
57
705
MIT-6-R
AAGCCTCCTATGATGGCAAA
 
 
MIT-7-F
GCCATCATAGGAGGCTTCATT
59
702
MIT-7-R
TTTCCTGAGCGTCTGAGATGT
 
 
MIT-8-F
CATCTCAGACGCTCAGGAAA
55
730
MIT-8-R
GGGAGGTAGGTGGTAGTTTGTG
 
 
MIT-9-F
TACTACCGTATGGCCCACCA
60
781
MIT-9-R
AGGCTTGGATTAAGGCGACA
 
 
MIT-10-F
ACTGACTATCCTAGAAATCGCTGT
59
741
MIT-10-R
GCAGATAGTGAGGAAAGTTGAGC
 
 
MIT-11-F
CCACGGACTTCACGTCATTA
59
743
MIT-11-R
GGGAGGATATGAGGTGTGAGC
 
 
MIT-12-F
GCATTTACCATCTCACTTCTAGG
57
751
MIT-12-R
AGTGCGATGAGTAGGGGAAG
 
 
MIT-13-F
AGGCACATACTTCCTATTCTACACC
55
740
MIT-13-R
TGATATTTGATCAGGAGAACGTG
 
 
MIT-14-F
ACTGGGAGAACTCTCTGTGCT
55
740
MIT-14-R
ACGAACAATGCTACAGGGATG
 
 
MIT-15-F
TCCATAATATTCATCCCTGTAGCA
57
741
MIT-15-R
GTGGGTACAGATGTGCAGGA
 
 
MIT-16-F
GTTACAATCGGCATCAACCA
55
740
MIT-16-R
AGCTTTTCTAGTCAGGTTAGGTCTA
 
 
MIT-17-F
CCTCAACCCAAAAAGGCATA
59
745
MIT-17-R
GGAGGTCGATGAATGAGTGG
 
 
MIT-18-F
ACGCAAAATTAACCCCCTAA
59
742
MIT-18-R
GCCTAGGAGGTCTGGTGAGA
 
 
MIT-19-F
CCTAGGCGACCCAGACAAT
59
740
MIT-19-R
GATAGTTGAGGGTTGATTGCTGTA
 
 
MIT-20-F
TGCTTACAAGCAAGTACAGCAAT
57
370
MIT-20-R
TGATGTCTTATTTAAGGGGAACG
 
 
MIT-21-F
GGCTCACATCACCCCATAAA
55
720
MIT-21-R
CATGGGCTACACCTTGACCT
 
 
MIT-22-F
GCAAACCCTGATGAAGGCTA
57
632
MIT-22-R
GGGGTCTTAGCTTTGGCTCT
 
 
MIT-23-F
ACTTTGCAAGGAGAGCCAAA
59
672
MIT-23-R
AGGCGGTGCCTCTAATACTG
 
 
MIT-24-F
TCACCTCTAGCATCACCAGTATT
57
660
MIT-24-R
GGAAGGCGCTTTGTGAAGTA
 
 
HVS-I-F
ACT TCA CAA CAA TCC TAA TCC T
60
1200
HVS-I-R
CGG AGC GAG GAG AGT AGC AC
 
 
HVS-II-F
CAT TTA CCG TAC ATA GCA CA
60
820
HVS-II-R GGG AGG GGG TGA TCT AAA AC