Table 2. Primers used for mitochondrial haplogrouping.
Primer # | Primer sequence | Annealing temp. | Product size |
---|---|---|---|
MIT-1-F |
CCTCCCTGTACGAAAGGACA |
57 |
614 |
MIT-1-R |
TGAGATTGTTTGGGCTACTGC |
|
|
MIT-2-F |
CGAGCAGTAGCCCAAACAAT |
57 |
664 |
MIT-2-R |
TTTTGGATTCTCAGGGATGG |
|
|
MIT-3-F |
CCATCCCTGAGAATCCAAAA |
57 |
653 |
MIT-3-R |
ATTTTGCGTAGCTGGGTCTG |
|
|
MIT-4-F |
TAAACCAGACCCAGCTACGC |
55 |
661 |
MIT-4-R |
AAAGTGGCTGATTTGCGTTC |
|
|
MIT-5-F |
TCAACTGAACGCAAATCAGC |
60 |
784 |
MIT-5-R |
TGAAATTGATGGCCCCTAAG |
|
|
MIT-6-F |
CTTAGGGGCCATCAATTTCA |
57 |
705 |
MIT-6-R |
AAGCCTCCTATGATGGCAAA |
|
|
MIT-7-F |
GCCATCATAGGAGGCTTCATT |
59 |
702 |
MIT-7-R |
TTTCCTGAGCGTCTGAGATGT |
|
|
MIT-8-F |
CATCTCAGACGCTCAGGAAA |
55 |
730 |
MIT-8-R |
GGGAGGTAGGTGGTAGTTTGTG |
|
|
MIT-9-F |
TACTACCGTATGGCCCACCA |
60 |
781 |
MIT-9-R |
AGGCTTGGATTAAGGCGACA |
|
|
MIT-10-F |
ACTGACTATCCTAGAAATCGCTGT |
59 |
741 |
MIT-10-R |
GCAGATAGTGAGGAAAGTTGAGC |
|
|
MIT-11-F |
CCACGGACTTCACGTCATTA |
59 |
743 |
MIT-11-R |
GGGAGGATATGAGGTGTGAGC |
|
|
MIT-12-F |
GCATTTACCATCTCACTTCTAGG |
57 |
751 |
MIT-12-R |
AGTGCGATGAGTAGGGGAAG |
|
|
MIT-13-F |
AGGCACATACTTCCTATTCTACACC |
55 |
740 |
MIT-13-R |
TGATATTTGATCAGGAGAACGTG |
|
|
MIT-14-F |
ACTGGGAGAACTCTCTGTGCT |
55 |
740 |
MIT-14-R |
ACGAACAATGCTACAGGGATG |
|
|
MIT-15-F |
TCCATAATATTCATCCCTGTAGCA |
57 |
741 |
MIT-15-R |
GTGGGTACAGATGTGCAGGA |
|
|
MIT-16-F |
GTTACAATCGGCATCAACCA |
55 |
740 |
MIT-16-R |
AGCTTTTCTAGTCAGGTTAGGTCTA |
|
|
MIT-17-F |
CCTCAACCCAAAAAGGCATA |
59 |
745 |
MIT-17-R |
GGAGGTCGATGAATGAGTGG |
|
|
MIT-18-F |
ACGCAAAATTAACCCCCTAA |
59 |
742 |
MIT-18-R |
GCCTAGGAGGTCTGGTGAGA |
|
|
MIT-19-F |
CCTAGGCGACCCAGACAAT |
59 |
740 |
MIT-19-R |
GATAGTTGAGGGTTGATTGCTGTA |
|
|
MIT-20-F |
TGCTTACAAGCAAGTACAGCAAT |
57 |
370 |
MIT-20-R |
TGATGTCTTATTTAAGGGGAACG |
|
|
MIT-21-F |
GGCTCACATCACCCCATAAA |
55 |
720 |
MIT-21-R |
CATGGGCTACACCTTGACCT |
|
|
MIT-22-F |
GCAAACCCTGATGAAGGCTA |
57 |
632 |
MIT-22-R |
GGGGTCTTAGCTTTGGCTCT |
|
|
MIT-23-F |
ACTTTGCAAGGAGAGCCAAA |
59 |
672 |
MIT-23-R |
AGGCGGTGCCTCTAATACTG |
|
|
MIT-24-F |
TCACCTCTAGCATCACCAGTATT |
57 |
660 |
MIT-24-R |
GGAAGGCGCTTTGTGAAGTA |
|
|
HVS-I-F |
ACT TCA CAA CAA TCC TAA TCC T |
60 |
1200 |
HVS-I-R |
CGG AGC GAG GAG AGT AGC AC |
|
|
HVS-II-F |
CAT TTA CCG TAC ATA GCA CA |
60 |
820 |
HVS-II-R | GGG AGG GGG TGA TCT AAA AC |