Table 1.
Summary of the primary and secondary antibody combinations and concentrations used in the current study
| Primary antibody combination | Primary antibody concentration | Supplier | Secondary antibody combination | Secondary antibody concentration | Supplier | |
|---|---|---|---|---|---|---|
| Cat | Guinea pig/rabbit VGLUT1 | 1:5000 | Chemicon/Synaptic systems | Cy-5 | 1:100 | Jackson ImmunoResearch |
| Rabbit VGLUT2 | 1:5000 | Chemicon | Alexa 488 | 1:500 | Molecular Probes | |
| Rat | Mouse CTb | 1:250 | A. Wikström, University of Gothenburg | Rh. Red | 1:100 | Jackson ImmunoResearch |
| Guinea pig VGLUT1 | 1:5000 | Chemicon | Cy-5 | 1:100 | Jackson ImmunoResearch | |
| Rabbit VGLUT2 | 1:5000 | Chemicon | Alexa 488 | 1:500 | Molecular Probes |
All secondary antibodies were raised in donkey and conjugated to: Rh. Red, Rhodamine Red; Cy-5, Cyanine 5.18; Alexa 488, Alexa Fluor 488; CTb, b-subunit of cholera toxin; VGLUT, vesicular glutamate transporter.