Table 1.
Bacterial strains and plasmids used in this study
| Strains/plasmid | Description | References |
|---|---|---|
|
E. coli |
|
|
| Top 10 |
F-mcrA (mrr-hsdRMS-mcrBC) F80 lacZ M15 lacX74 recA1 ara 139(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG |
Invitrogen |
|
SalmonellaGallinarum |
|
|
| JOL394 |
Salmonella Gallinarum, isolated from chicken |
This study |
| JOL 1277 |
Salmonella Gallinarum JOL394 containing pMMP99 |
This study |
|
Plasmids |
|
|
| T-vector |
Cloning vector; pUCori AmpR |
Promega |
| pMMP99 | A derivative of T-easy carrying ghost cassette | This study |