Table 4.
Mortality and gross lesion in the chickens post challenge
| Groupa |
Challengeb |
||||
|---|---|---|---|---|---|
|
Gross lesiond |
Bacterial recovery |
||||
| Mortality (%) | Liver | Spleen |
Liver |
Spleen |
|
| Positive No. | Positive No. | ||||
| A |
11/15 (73)c |
2. 2.16 ± 1.46e** |
1.70 ± 1.46** |
0/5f |
0/5 |
| B |
2/15 (14) |
1.06 ± 0.96 |
0.66 ± 1.12 |
0/5 |
0/5 |
| C |
1/15 (6.6) |
0.60 ± 0.98 |
0.53 ± 0.83 |
0/5 |
0/5 |
| D | 1/15 (6.6) | 0.53 ± 0.89 | 0.53 ± 0.93 | 0/5 | 0/5 |
a Immunization was performed at day 7 of age with the Salmonella Gallinarum ghost strain and the groups were designated as group A (non vaccinated), group B (orally immunized), group C (immunized subcutaneously) and group D (immunized intramuscularly). Fifteen birds were allocated per group.
b Challenge was performed with a wild type Salmonella Gallinarum strain using 1 × 106 CFU after 21 days post vaccination.
c Number of dead birds upon challenge.
d Gross lesion was observed at day14 post challenge.
e Group lesion score (Mean ± SD).
f Number of positive samples after enrichment culture. All values were considered to be significant if p ≤ 0.05 or 0.01.* p < 0.05, **p < 0.01 vs. non vaccinated group A.