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Characterization of isolated endothelial progenitor cells. Flow cytometric analysis of surface markers of EPCs using FITC-conjugated anti-CD146, PE-conjugated anti-CD34 and PE-Cy5-conjugated anti-CD45 monoclonal antibodies demonstrated that 78% (A) and 80% (B) of the cells were triple positive for the markers. FLI, FL2 and FL3 represent CD146, KDR and CD45 expressive cells, respectively. The percentage of expressing cells was calculated using the cell quest program.
