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. 2012 Jun 6;12:97. doi: 10.1186/1471-2180-12-97

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Copyright ©2012 Sabala et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Pulldown assay of various LytM fragments and inhibitors with purified peptidoglycans fromS. aureus. (A) Full length LytM and various fragments were analyzed by denaturing gel electrophoresis and Coomassie straining either directly (control, C) or after separation into peptidoglycan binding (PG) and supernatant (S) fractions. (B) LytM_185-316 was incubated with peptidoglycan in the presence of various protease inhibitors and the pellet fraction after pulldown analyzed by denaturing gel electrophoresis and Western blotting.