Figure 1. Beta-galactosidase activity in M. smegmatis expressing lacZ under the control of the promoter regions of the M. tuberculosis H37Rv lipF, pks2, msl3 and fadD21 genes.
The lipF, pks2, msl3 and fadD21 promoter-lacZ fusions carried by the pJEM15 plasmid were introduced into M. smegmatis. The positive control is M. smegmatis transformed with pJEM31(PAN) containing lacZ under the control of a mobile genetic element normalized against M. smegmatis transformed with pJEM15 empty vector. The results shown are the mean of two independent experiments.