Table 3. Identification of the DR1–3 sites on the DNA promoters recognized byPhoP.
DR1 | DR2 | DR3 | |
msl3 | TCTGGTAGC 8/9: 89% | CATGGCAAC 7/9: 78% | AATGTGTTC 5/9: 56% |
fadD21 | TGTTTCAGC 7/9: 78% | ATGCACAGC 6/9: 67% | ATGATCAGC 7/9: 78% |
lipF | ACGTACAGC 8/9: 89% | ACTCCCAGT 6/9: 67% | CCTGTGATC 6/9: 67% |
pks2 | CCCAGTAGC 6/9: 67% | CCGCTTAGA 6/9: 67% | AGACACAGC 5/9: 56% |
phoP | ACTGTTAGC 9/9: 100% | ACTGGCAAC 9/9: 100% | - |
consensus sequence of phoP | A C T/G T/G T/G Py A Pu C |
For each DNA sequence shown to bind PhoP-P, sequences with a complete or partial match to the DR1, DR2 and DR3 sites were identified (underlined). The degree of conservation of the consensus sequence was calculated and is shown as the ratio of matching nucleotides to the consensus: % identity.