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. 2012 Aug 7;7(8):e42417. doi: 10.1371/journal.pone.0042417

Figure 3. Specific in vitro Binding of Vimentin to Promoter Variants.

Figure 3

(A) Biotin-labeled duplex oligos and 10 µg NIH3T3 nuclear extract were used for the experiments. Arrow indicates protein binding to the oligos. (B) Determination of specificity of duplex 29G* by completion with unlabeled duplex oligos. (C) Specific binding of vimentin to 29G* and 29A* oligos was demonstrated with anti-vimentin antibody.