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. 2012 Aug 7;7(8):e42434. doi: 10.1371/journal.pone.0042434

Figure 2. Coomasie blue stained SDS-PAGE gel of purified recombinant TcpF-A2-CTB chimera and TcpF proteins.

Figure 2

Samples were reduced and boiled prior to loading on a 15% SDS-PAGE gel, causing the TcpF chimera to separate into the larger TcpF-A2 (∼42 kDa) fusion protein and the monomeric CTB proteins (11.5 kDa).