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. 2012 Aug 7;7(8):e42894. doi: 10.1371/journal.pone.0042894

Figure 1. HSP12 differs among yeast species.

Figure 1

(A) Two CaHSP12 genes have been identified in C. albicans SC5314 and five clinical isolates by Southern blot. (B) Both alleles of CaHSP12 are transcriptionally expressed. The transcription level of CaHSP12 is assessed by qRT-PCR of total RNA obtained from the strain with absence of one CaHSP12 gene (HSP12KO2) and its parental strain (BWP17). The error bars represent the S.D. of triplicate independent reactions. P value<0.01, two-sided unpaired student t-test. (C) 5′ RACE analysis of CaHSP12. Two DNA bands (band 1 and band 2) with the expected size above 250 bp were sequenced. The sequencing shows that the 5′ untranslated region (in lowercase) contains 29 bp nucleotides and only the second start codon (underlined) of CaHSP12 can be identified. M: 1 kb DNA ladder; -ve: negative control of PCR without template; HSP12: 5′RLM-RACE PCR product of CaHSP12.