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. 2012 Aug 7;7(8):e42894. doi: 10.1371/journal.pone.0042894

Figure 2. Hsp12p is regulated in response to a wide range of stresses.

Figure 2

(A) CaHsp12p is recognized by a polyclonal antibody. The anti-CaHsp12p antibody was tested by using Western blot analysis against protein samples from the C. albicans CAI4 control strain (Ct) and the Cahsp12 null mutant (Δhsp12). The arrow indicates the 13 kDa band of CaHsp12p which is present in CAI4, but absent in the Cahsp12 null mutant. (B) Induction of Hsp12p in C. albicans and in S. cerevisiae during stationary growth. Total protein was extracted at the indicated time points from C. albicans CAI4 at 37°C or S. cerevisiae BY4741 at 30°C. Western blots were probed with anti-Hsp12p antibody and showed a band corresponding to the expected size of 13 kDa. Blots were probed with anti-actin antibody as loading control. Growth curves with sampling time points (open or solid dots) are shown. RDU: relative densitometry units. (C) Hsp12p is regulated in response to diverse conditions. Hsp12 protein level in C. albicans CAI4 or S. cerevisiae BY4741 was assayed using Western blot and a band of the expected size (13 kDa) was detected. H: anti-Hsp12p antibody. A: anti-actin antibody (equal protein loading control). RDU: relative densitometry units. CgHSP12 transcript level was determined by qRT-PCR with total RNA extracted from the Cg2001TU strain. The transcript level was normalized to the Act1 control. The error bars represent the S.D. of triplicate independent reactions. **P value<0.01, * P value>0.3, two-sided unpaired student t-test.