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The binding of shFcRn-bound m2a1 to sp62 was measured at pH 6.0 (▪) and pH 7.4 (□) by ELISA. m2a1 could still bind to sp62 after binding to shFcRn at pH 6.0. Binding of shFcRn-bound Fc to sp62 at pH 6.0 (•) and pH 7.4 (○) was used as control. Data presented as mean ± SD.
